Fig. 7.

Determination of the optimal ratio of activating versus co-stimulating CriBs-Ab. In a proliferation assay, T cells were co-incubated with a criss-cross serial dilution of both CriBs-Abs (0–80 nM) in a pretargeting setting performed in a 96 well plate, in the absence (a) or presence (b) of mitomycin treated tumor cells. A separate graph was made from the same data displayed in a and b, at a constant concentration of 80 nM co-stimulating CriBs-Ab and a serial dilution (0–80 nM) of the activating CriBs-Ab, in the absence (open traingle) or presence (open square) of mitomycine treated tumor cells (c)