Fig. 3.

a IL-12 p40 secretion by captured Mo-DCs in the presence of anti-TNF-α neutralizing antibody (1 μg/ml). Mo-DCs (10⁴) were cocultured with 1×10⁵ dead GCTM-1 cells for 24 h. Cell-free culture supernatants were collected, and the concentration of of IL-12 p40 was measured by ELISA. Data are mean ± SE (bars). b IL-12 p40 secretion by captured Mo-DCs in the presence of recombinant TNF-α (200 U/ml). Mo-DCs (10⁴) were cocultured with 1×10⁵ dead GCTM-1 cells for 24 h. Cell-free culture supernatants were collected, and the concentration of of IL-12 p40 was measured by ELISA. Data are mean ± SE (bars). c TNF-α secretion by Mo-DCs-Tum in the presence of anti-IL-12 (20 μg/ml). Mo-DCs (10⁴) were cocultured with 1×10⁵ dead GCTM-1 cells for 24 h. Cell-free culture supernatants were collected, and the concentration of TNF-α was measured by ELISA. Data are mean ± SE (bars). d TNF-α secretion by captured Mo-DCs in the presence of recombinant IL-12 (100 μg/ml). Mo-DCs (10⁴) were cocultured with 1×10⁵ dead GCTM-1 cells for 24 h. Cell-free culture supernatants were collected, and the concentration of TNF-α was measured by ELISA. Data are mean ± SE (bars). The data are representative of three independent experiments using Mo-DCs generated from ten different donors