Figure 1.

HBCs at 24 hpi increase apical expression of Itgb1 and Itgb4

(A–D) Representative immunofluorescence images demonstrating that relative to HBCs within uninjured (uninj.) OE (A, B), HBCs at 24 hpi are activated as they demonstrate p63 downregulation (C, D).

(E) Quantification of nuclear p63 normalized (norm.) fluorescence (fluor.) density within CK5⁺ HBCs; each triangle represents a CK5⁺ Nuclear Object (Obj.) (blue outlines in B and D) used to quantify the encompassed p63 fluorescence.

(F–M) Representative immunofluorescence images demonstrating that HBCs at 24 hpi (J–M) increase apical Itgb1 (within green outlines, I and M) relative to HBCs within uninj. OE (F–I).

(N) Quantification of Itgb1 norm. fluor. density within HBC apical domains; each triangle denotes an analyzed region as represented in (I) and (M) (n = 19 regions across 3 mice).

(O–V) Representative immunofluorescence images demonstrating that HBCs at 24 hpi (S–V) increase apical Itgb4 (within green outlines, R and V) relative to HBCs within uninj. OE (O–R).

(W) Quantification of Itgb4 norm. fluor. density within HBC apical domains; each triangle denotes an analyzed region as represented in (R) and (V) (n = 17 regions across 3 mice). Unpaired t test (E, J), Mann-Whitney test (O). Uninj. used as baseline, error bars indicate mean ± SEM, ∗∗p < 0.01, ∗∗∗∗p < 0.0001 (E, N, W). Scale bar equals 10 μm (A).