Primary HBCs activated in vitro enhance Rac1-mediated signaling
(A) Representative western blots depicting p63, Rac1, pcJun, total cJun, and GAPDH abundances in cultures of vehicle-treated (PMA-/EHT1864-), activated (PMA+/EHT1864-), activated and Rac1 inhibited (PMA+/EHT1864-), and Rac1 inhibited (PMA-/EHT1864+) HBCs.
(B–D) Quantification of densitometric measurements for p63 (B), Rac1 (C), and normalized pcJun per normalized total cJun (D) abundances (n = 3 independent trials), vehicle-treated used as baseline, error bars indicate mean +SEM, one-way ANOVA with post hoc Tukey’s multiple comparisons test, ∗<0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.