Figure 5.

Macrophages express aqueous-enriched chemokines, including CCL2 and CXCL10. A, Violin plots mRNA expression of chemokines by aqueous immune cells. Gene expression measured by single cell RNA sequencing was pseudobulked by patient, and each dot represents a single patient. B, Scatter plots comparing mRNA expression by cell type indicated in parentheses versus protein abundance in aqueous fluid. Transcript expression was pseudobulked by patient, and protein concentrations were logarithmized. Each point represents a single patient. We assessed for correlation using linear regression and plotted the 95% confidence interval. Statistical significance is indicated as follows: ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001. CCl = C-C motif chemokine ligand; cDC = conventional dendritic cell; CXCL = C-X-C motif chemokine ligand; NK = natural killer; pDC = plasmacytoid dendritic cell.