Figure 6.

Macrophage-expressed chemokines can signal to all aqueous immune cell types. Potential ligand-receptor pairs were identified based on cell type-specific gene expression using SingleCellSignalR. A, Alluvial diagram showing high number of predicted interactions (wide bands) between macrophage-expressed ligands and receptors expressed by other cells. Cell types expressing ligand genes are on the left and cell types expressing receptor genes are on the right. The thickness of the connecting bands indicates the number of ligand–receptor interactions between the 2 connected cell types. B, Network plot showing strong predicted ligand–receptor interaction (LR score, line color) between macrophage ligands (purple diamonds) and receptors (chevrons) expressed by other aqueous cell types. Cell types are indicated by the color of the icon. Chevron icons indicate receptor genes and diamond icons indicate ligand genes. CCL = C-C motif chemokine ligand; CCR = C-C motif chemokine receptor; cDC = conventional dendritic cell; CXCL = C-X-C motif chemokine ligand; NK = natural killer; pDC = plasmacytoid dendritic cell.