Fig. 1.

DOX increases m⁶A modification and METTL3 expression in cardiomyocytes. (A)-(C) Representative m⁶A dot blot and statistical analysis of m⁶A level in DOX-treated HL-1 cells, neonatal rat ventricular cardiomyocytes (NRCMs), and mouse heart tissues. (D) and (E) Representative immunoblots and statistical analysis of protein expression of m⁶A methyltransferases and demethylases. (F) Representative immunohistochemical staining of METTL3 in hearts from a mouse model of DOX-induced cardiotoxicity. (G) Representative immunofluorescence staining of METTL3 in DOX-treated HL-1 cardiomyocytes. Mean ± SEM, n = 6 per group. P values were determined by unpaired two-tailed Student's t-test (A-C and E).*P < 0.05, and **P < 0.01.