FIG. 2.

Growth in soft agar of neoR-transfected, EBV-reinfected, and EBNA-1-transfected Akata cell clones. Three sets of clones originating from four independent EBV-negative Akata clones were examined in this study (A, B, C, and D). EBV-reinfected cells could form colonies, but neoR-transfected and EBNA-1-transfected clones could scarcely do so. It is clear that EBNA-1 is not solely responsible for the growth of Akata cells in soft agar. Each dot represents the number of visible colonies of a single clone formed in soft agar. A total of 10⁴ cells were seeded per well. The number of clones tested is noted underneath the graph. Values between the indicated groups (Mann-Whitney U test): ∗, P < 0.005; ∗∗, P < 0.05; ∗∗∗, P < 0.01.