Figure 4.

SB induced cell cycle arrest trough upregulation of p21 and downregulation of CDK1. CD90 surface expression increased upon SB treatment. (A) Schematic representation of cell cycle regulating proteins influenced by SB. SB leads to a lower gene expression of the cyclin-dependent kinases (CDKs), which are required for the normal cell cycle (green). The counterpart of the CDKs are kinase inhibitors, like p21, p27 and p16 (red). These inhibitors stopping the cell cycle at each checkpoint and therefore the proliferation of the cell; (B) qPCR analysis of relative CDKN1A(p21)/RPL30 mRNA expression in SB treated A549, (n=4); (C) qPCR analysis of relative CDK1/RPL30 mRNA expression in SB treated A549, (n=4); (D) qPCR analysis of relative CDKN1A/RPL30 mRNA expression in SB treated A549, (n=4); (E) A549 cells treated with SB were stained with anti-CD90 antibody and analyzed with flow cytometry, (n=6); (*P < 0.05; **P < 0.01; ****P < 0.0001). One-way ANOVA test was used for figure (D, F), Kruskal-Wallis test was used for figure (C, E). All data are presented as mean values ± SEM.