FIG. 7.

Virus release and reactivation in persistently infected cell lines. (A) Productive viral infection of Sf9 cells as a control. Sf9 cells were infected with wild-type AcMNPV (lane 1) and vAcZΔp35 (lane 2) at an MOI of 10. Lane 3 is the virus yield of Sf9 cells first transfected with plasmid pki^h35hN and then infected with vAcZΔp35. (B) Release and reactivation of viruses in persistently infected cells. Lanes: 1, 3, and 5, continuous virus-releasing levels of different persistently infected cells; 2, 4, and 6, yields of viruses from these persistently infected cells transfected with pKi^h35hN. All persistently infected cells were analyzed at 80 to 100 passages.