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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Nat Protoc. 2023 Nov 1;18(12):3732–3766. doi: 10.1038/s41596-023-00893-w

Figure 16 |. In vivo experiment workflow.

Figure 16 |

a, Schematics of the guide star (Cy5.5-dextran) delivery by retro-orbital injection. b, Schematic diagram of the AO correction. Spot patterns formed by the SHWS from the descanned guide star signal in the capillary, together with the sample reference pattern and AO calibration results of the DM Zernike modes, are used for calculating the DM pattern for correcting the sample aberration. The sample aberration correction pattern is added to the system aberration pattern for full AO correction.