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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Nat Protoc. 2023 Nov 1;18(12):3732–3766. doi: 10.1038/s41596-023-00893-w

Figure 18 |. Functional imaging AO correction.

Figure 18 |

a, In vivo two-photon imaging of the layer 5 neurons of vS1 cortex which were labelled with jRGECO1a. Data were obtained at 630 μm below the pia with full AO correction using excitation wavelength λ = 1030 nm. b, Calcium signal in the ROIs defined by the dashed circles in panel a. c, In vivo two-photon imaging of the thalamocortical boutons which were labelled with iGluSnFR3. Data were obtained at layer 4 of vS1 cortex with full AO correction using excitation wavelength λ = 970 nm. d, Glutamate dynamics responding to 5 Hz air puff in the ROIs defined by the dashed circles in panel c. The trace is averaged from 60-s recording.