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. Author manuscript; available in PMC: 2024 Dec 1.
Published in final edited form as: Nat Protoc. 2023 Nov 1;18(12):3732–3766. doi: 10.1038/s41596-023-00893-w

Figure 5 |. Overview of the procedure.

Figure 5 |

Flowchart illustrating the essential steps for constructing, aligning, and calibrating the AO-TPLSM as well as showing the main steps for conducting an imaging experiment with AO-TPLSM.