FIG. 3.
RSK1 activity during HCMV infection. RSK1 activity was determined by an immune complex kinase assay. RSK1 was immunoprecipitated from serum-starved cell lysates and mock- or virus-infected cell lysates at the indicated times postinfection. RSK1 activity was detected by using an S6 kinase assay kit (Upstate Biotechnology) and [γ-32P]ATP. Bars represent the average of two independent assays depicted as counts per minute of 32P-labeled peptide bound to filters as quantitated by scintillation counting; error bars represent half of the range of the two values.