FIG. 2.

Localization of viral antigen and phenotypically distinct populations of leukocytes in the oculomotor nucleus after injection of PRV Becker into the vitreous body of the eye. At the top of the figure are schematic drawings (modified from Swanson’s brain map computer graphics files [59]) of a sagittal view of the brain (left) and a coronal section with an open square in the middle indicating the location of the oculomotor nucleus (right). Micrographs of adjacent sections of tissue that were processed with antibodies recognizing viral, CD45, ED-1, or CDβ⁺ antigen are shown below the drawings. (A to D) Micrographs of brain tissue from a representative rat with several virus-infected neurons. These pictures show viral immunoreactivity in the oculomotor nucleus (A), immunoreactive cells expressing the CD45 antigen and an enlarged view of these cells by a blood vessel (B), ED-1⁺ cells (C), and CD8β⁺ leukocytes (D). (E to H) Micrographs of brain tissue from a representative rat with many virus-infected neurons in the oculomotor nucleus. This series of micrographs show viral immunoreactivity (E), immunoreactive cells expressing the CD45 antigen (F), ED-1⁺ immunoreactive cells (G), and CD8β⁺ leukocytes (H). Scale bar, 100 μm for all figures. Arrows indicate blood vessels.