Fig. 6.

Gβγ and Gq inhibition enhance morphine-induced antinociception in mice. (A) Mice were injected intracerebroventricularly with gallein (100 nmoles) or DMSO (8 mice per condition) and allowed to recover for 30 minutes, and post-intracerebroventricular tail flick latency was measured. Then 3.2 mg/kg of morphine was injected at time 0, and tail flick latency was measured at the indicated times; mixed effects 2-way ANOVA with Sidak’s multiple comparisons, significant interaction of time X pretreatment effect F(5,69) = 9.2, P ≤ 0.0001 (B) Same as (A) except mGα_q-CT (5 male mice) or myrGα_q-scrambled (4 male mice) were injected intracerebroventricularly at 30 nmoles each; mixed effects 2-way ANOVA with Sidak’s multiple comparisons, significant interaction of time X pretreatment effect F(5,35) = 2.78, P = 0.03. Data were analyzed with a mixed effects ANOVA followed by Sidak’s multiple comparisons test. *P < 0.05, **P < 0.005, ****P < 0.0001 at each time point comparing treatment to control (gallein vs. DMSO or mGα_q-CT vs. Scr peptide). Data are mean ± S.D.