Fig. 7.

The TOP2α/90 isoform is expressed in human tissues and bone marrow cells. Representative TOP2α immunoblotting experiments using the indicated tissue or cellular lysates. The immunoblots were probed with TOP2α/90/170 Ab or β-actin Ab. Immunoblots were subsequently incubated with a secondary antibody, and cross-reactive proteins were detected using chemiluminescence (Kanagasabai et al., 2017). The following amount of protein was loaded into each well: K562 cellular lysate, 7 µg; K/VP.5 cellular lysate, 7 µg; human cord blood mononuclear cell (MNC) lysate, 30 µg; AML patient blast cell lysates (pretreatment and after relapse), 20 µg; normal human stomach tissue lysate, 15 µg; normal human thymus tissue lysate, 15 µg.