Fig. 3.
Characterization of striatal D2 receptors. Results are shown for [3H]spiperone binding to striatal membranes from 11–13 month old Drd2+/+ (WT), Drd2+/I212F (Het), and Drd2I212F/I212F (I212F) mice. Saturation binding analysis was carried out using the D2 antagonist radioligand [3H]spiperone, and the Bmax and affinity of D2 receptors for the radioligand (Kd) was obtained by nonlinear regression. (A) Representative binding curves from one mouse of each genotype. (B) Mean Bmax values ± S.D. (C) Mean Kd values ± S.D. *P < 0.05; *** P < 0.001; **** P < 0.0001 by Tukey’s post hoc test.