Skip to main content
. 2004 Jan 13;53(6):533–542. doi: 10.1007/s00262-003-0460-1

Fig. 4a–f.

Fig. 4a–f

Autoradiography was done on 6-μm serial sections of xenografts of LNCaP-LN3 and DU-145, fresh-frozen in OCT, 7 days after i.p. injection of 125I-labeled MAbs (BLCA-38 or J591, respectively) or 2 days after injection of appropriate 125I-labeled F(ab’)2s or Fabs. Control slides were xenograft tissue from mice that had not been injected with antibodies. Thawed slides were fixed in 1% paraformaldehyde (Sigma, St. Louis, MO), dipped in Emulsion (Kodak NTB-3, Integrated Sciences) at 1 g emulsion/ml of glycerol solution in a darkroom lit with an Ilford 915 red light filter and then exposed for 7–14 days at 4°C before being developed in freshly prepared developer, dried and stained with H&E. Autoradiographs of fresh-frozen xenografts: a DU-145 xenograft tissue, negative control; b DU-145 xenograft 7 days after injection of 125I-labeled BLCA-38 MAbs; c DU-145 xenograft 2 days after injection of 125I-labeled BLCA-38 F(ab’)2; d DU-145 xenograft 2 days after injection of 125I-labeled BLCA-38 Fab; e LNCaP-LN3 xenograft, negative control; f LNCaP-LN3 xenograft, 7 days after injection of 125I-labeled J591 MAb