Fig. 7A,B.

Peptide-specific Th response induced by immunization with CEA₁₁₆. The HLA-DR4 transgenic mice (4 mice/group) were twice immunized with 100 μg CEA₁₁₆ or phosphate-buffered saline (PBS; 100 μl) emulsified with complete Freunds adjuvant (CFA). Ten days after the second immunization, the splenocytes of each mouse group were pooled and tested for peptide CEA₁₁₆-induced production of IFN-γ by ELISPOT. A The frequency of peptide-specific T-cells of CEA₁₁₆-immunized mice was significantly higher than that of PBS control group and irrelevant control peptide CEA₅₈₈ (p<0.01), and B significantly decreased by monoclonal antibody anti-human CD4 (p<0.01). Data show the mean and SD of spot numbers from triplicate wells from one experiment that is representative of two experiments performed