Fig. 3.
IL-2 secretions of splenocytes restimulated with irradiated B16-MAGE-1 were measured using ELISA. C57BL/6 mice were vaccinated as described in Fig. 1. The splenocytes were pooled 2 weeks after the booster. Splenocytes (4×106) were cocultured with 5×105 irradiated B16-MAGE-1 cells for 3 days in a final volume of 2 ml, and the supernatants were obtained and the concentrations of IL-2 were determined using murine IL-2 ELISA kits. Statistical analysis by a paired Student’s t-test demonstrated that the IL-2 concentration in supernatants of splenocytes from mice vaccinated with MAGE-1-HSP70 fusion DNA was higher than that from mice with MAGE-1 DNA (p<0.05, n=6), or with a mixture of MAGE-1 DNA and HSP70 DNA (p<0.05, n=6)