Fig. 2.
Reduced disseminated melanoma metastases in mice injected intravenously with B16αGal cells. Female and male αGT KO mice were immunized with RRBC to increase the anti-αGal Ab titers. One week after the last immunization, mice were intravenously injected with B16NeoR (n=7) or B16αGal (n=11) in the tail vein. Three weeks after the injection, lungs were harvested and lung melanoma metastases were enumerated in a blinded manner. Results indicate that the difference in lung burden is statistically significant (t test P<0.05. Bars show the average and standard error of the mean (SEM)