Fig. 2A, B.
Effect of α-TOS on established 3LL tumors in vivo. Mice were injected s.c. with 106 3LL tumor cells. On day 10, established tumors (~30 mm3) were injected i.t. or i.p. with α-TOS, NaS, or ethanol. Injections (4-mg α-TOS) were given (depicted by double arrows on x-axis) on days 10, 13, and 16. A Data are mean tumor volumes ± SEM. B For the TUNEL assay, mice with preestablished tumors were injected with α-TOS on days 10, 14, and 18. Forty-eight hours after each α-TOS injection, tumors were resected, frozen, sectioned, and stained with the TUNEL reaction mixture. The orange and green regions in the tumor sections represent TUNEL positive, and red cells depict TUNEL negative cells. Magnification ×400