Fig. 6.

Effect of combination treatment with α-TOS+DCs on IFN-γ secretion by splenic lymphocytes. Spleens were isolated on day 30 post–tumor injection from each of three mice in each treatment group and pooled. CD4⁺ and CD8⁺ T cells were isolated from spleens by immunomagnetic bead separation, restimulated in vitro with tumor lysate–pulsed, TNF-α–matured DCs in 24-well tissue culture plates for 48 h. The supernatants were collected and assayed by ELISA for the production of IFN-γ. Data are mean ± SEM of triplicate samples