Fig. 1.

Immunophenotype of monocyte-derived DCs generated in vitro from cells of patients or from healthy individuals. Peripheral blood monocytes from eight cancer patients and eight healthy individuals were cultured in the presence of GM-CSF plus IL-4 and activated with TNF-α on the 5th day of culture. On the 7th day, cells were harvested, and CD80, CD86, and CD83 surface expression was assessed by flow cytometry. Bars represent the mean % of positive cells for each marker; p values (healthy vs patients) were CD86, p=0.04; CD83, p=0.14; CD80, p=0.92.