Fig. 8.

Diminished Virulizin-mediated tumor infiltration of NK cells in macrophage-depleted mice. CD-1 nude mice harboring C8161 human melanoma xenografts were depleted of macrophages using liposome-Cl₂MDP, and then treated with saline or Virulizin (0.2 ml). Tumors were isolated from the mice at the end of the experiment. A cell suspension was prepared from tumors and stained with anti-DX5 (NK cell–specific) antibody conjugated with FITC. The number of NK cells in tumors were assessed by flow cytometry.