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Specific recognition of the K11-ABP fusion protein by mAb 225.28S in immunoblot. K11-ABP (lane 1) and ABP (lane 2) fusion proteins were subjected to 12% SDS-PAGE and blotted onto a nitrocellulose membrane. Strips were incubated with A an anti-His tag antibody, B mAb 225.28S, C isotype control, and D buffer. Bound antibodies were detected by a radioactively (125I) labeled sheep anti-mouse Ig antibody
