Fig. 3.

Sera of mice immunized with K11-ABP recognize the HMW-MAA band at 250 kDa in an 518A2 cell lysate immunoblot. a Immunoblot. 518A2 membrane preparations were separated by 6% SDS-PAGE and proteins were transferred to a nitrocellulose membrane. Strips were incubated with controls or pooled sera as indicated (b buffer control, neg isotype control, pos 225.28S, PIS preimmune serum, IS immune serum). b Immunization schedule. BALB/c mice (n=5 per group) were immunized ip with 10 μg of the respective fusion protein at the indicated time points (Imm immunization). Blood samples were taken 1 week after each immunization