Fig. 5.

Sera of mice immunized with K11-ABP induce specific lysis of 518A2 melanoma cells in an ADCC assay. 518A2 target cells were labeled with a substance fluorescent at contact with europium. Following incubation with 10 μg mAb/10 μl pooled sera (equaling ~2 μg HMW-MAA-specific antibodies), murine effector cells were added at an effector-to-target ratio of 50:1. Aliquots of supernatants were mixed with europium solution, and fluorescence was measured in a time-resolved fluorometer. Percentage of specific lysis was calculated relative to maximum release, corrected by spontaneous release values. Column heights represent mean ± SD. Effectors: lytic activity of effector cells alone. Other columns: ADCC mediated by mAb/pooled sera as indicated on the x-axis