Fig. 5.

Interferon-γ promotes tumor evasion of the immune system. a H22 tumor cells were treated with recombinant mIFN-γ at indicated concentration and harvested 3 days later and stained for PD-L1 expression. Mean fluorescence intensity (MFI) of the total stained cells is shown. b Mice (n=5 per group) were treated as in Fig. 3a and PD-L1, PD-L2, Foxp3 and CTLA-4 mRNA expression in peritumor tissues by day 20 after tumor cell inoculation were examined by relative quantitative real-time PCR. Data in a are representative of three separate experiments. Data in b are representative of two experiments