Fig. 2.

Immunisation of A2.1 K^b mice with DNApoly1 elicits epitope-specific memory CTL responses. Pooled splenocytes from mice (three per group) immunised twice with DNApoly1 DNA i.d. (a) or i.m. (b), or immunised once with an equimolar mix of epitope peptides (c), were restimulated with a single cognate peptide and reacted with EL4.A2 cells pulsed with the same peptide (open symbols) or without peptide (filled symbols), in a ⁵¹Cr-release cytotoxicity assay. Data points are means of triplicate tests ± standard deviation (SD). (Error bars, though plotted, do not appear, because SDs do not exceed symbol size). Data are from one of three experiments with similar results