Fig. 3.
Immunisation of HHD mice with DNApoly1 DNA elicits epitope-specific effector and memory CTL responses: a Pooled ex vivo splenocytes from mice (three per group) immunised twice i.d. with DNApoly1 were incubated with or without E7/A2/2 or flu/A2, peptide as indicated, and IFN-γ–secreting cells were quantified by ELISpot assay. b Pooled splenocytes from mice (three per group) immunised i.d. twice with DNApoly1 DNA, or with an equimolar mix of epitope peptides, were restimulated with a single cognate peptide in vitro and reacted with EL4.A2 peptide pulsed target cells (open symbols), or without peptide (filled symbols) as indicated in a 51Cr-release cytotoxicity assay. Data points are means of triplicate tests ± SD. (Error bars, though plotted, do not appear, because SDs do not exceed symbol size). Data are from one of two experiments with similar results