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Fusion efficiency. DCs and genetically engineered fibroblasts were stained with antimouse CD80 monoclonal antibody and PKH-26, respectively, and fused using PEG. Double-positive cells were determined to be fusion cells. A 85% of DCs were positive for anti-CD80 monoclonal antibody. B More than 97% of NIH/B16 cells were positive for PKH26. C The percentage of double-positive cells was 30.3%
