Fig. 1A–C.

Her-2/neu E75 and APL-specific responses detected from HLA-A2⁺ antigen normal donors by IFN-γ ELISpot assays and representative wells of IFN-γ ELISpot. T2 cells (2.5×10⁴ cells/well) pulsed with peptides (10.0 μg/ml) were used to stimulate bulk PBMC cultures (5×10⁴ cells/well). T2 cells were pulsed with the following peptides: E75 (green), APL 2 (yellow), APL 5 (pink), HBV (purple), unpulsed T2 cells (dark green), or CTL alone (blue). A CTLs raised on E75 (results from donor 3 were not included, as no IFN-γ-secreting, peptide-specific CTLs were detected). Representative spots from donor 5 shown to the right. B CTLs raised on APL 2 (representative spots from donor 4 shown to the right). C CTLs raised on APL 5 (representative spots from donor 1 shown to the right)