Fig. 3A–C.

Lysis of target cells pulsed with wild-type E75 peptide. CTLs (at the indicated E:T ratios) were incubated with ⁵¹Cr-labeled target cells (5×10³ cells/well), and ⁵¹Cr release from target cells was measured after 6–8 h. A E75-specific CTLs, B APL 2–specific CTLs, and C APL 5–specific CTLs lyse T2 cells pulsed with E75 (solid square). Lysis of the NK-sensitive cell line, K562 (open triangle), and lysis of T2 cells pulsed with HBV (open circle) served as background controls. Lysis of the MCF-7 breast cancer cell line (solid diamond) was also evaluated. Data are representative from donors 5, 4, and 1, respectively. Error bars represent the mean ± SEM of triplicate samples (*p<0.05)