Fig. 4.

Mixed lymphocyte reaction was up-regulated with DCs harvested from cancer-bearing mice treated with S-1 and/or LNT. C57BL/6 (H-2^b) and BALB/c (H-2^d) splenic T cells enriched by nylon wool column were used as responders. X-ray irradiated (20 Gy), MACS-sorted CD11c⁺ splenic DCs from cancer-bearing mice (BALB/c, H-2d) were cultured at graded concentrations with 2×10⁵/well responder T cells for 72 h. [³H]-Thymidine was added 18 h before harvesting. Data are expressed as mean ± SD of two separate experiments. There is a significant difference in [³H]-thymidine incorporation between CD11c⁺ splenic DCs originating from mice treated with S-1 alone and those originating from mice treated with S-1+LNT (*P<0.05). Data are representative of two independent experiments.