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. 2004 Sep 9;54(2):129–140. doi: 10.1007/s00262-004-0583-z

Fig. 2.

Fig. 2

HLA-A*02 restriction and lytic profile of KS24.22-stimulated CTL clone P2. The CTL clone P2 was generated by KS24.22 stimulation as described in “Materials and methods” and tested in a LDH release assay. a Cytolysis was determined on the KS24.22 transfectants (HLA-A*02+), either BB7.2 mAb pretreated (open circles) or not (filled circles), on KS24 transfectants (filled triangles), KS-mock transfectants (filled diamonds), on the parental cell line KS (filled squares), and on K562 (open squares). b The cytolytic profile of the CTL clone P2 was further analyzed using the breast cancer lines SkBr3.A2 (HLA-A*02+) (filled triangles) and AR (HLA-A*02−) (filled squares), as well as the colon carcinoma cell line SW480 (HLA-A*02+) (open triangles). KS24.22 killing was included as positive control (open squares).