Table 2.
Induction of MP57-68-specific T cells by allogeneic KS24 breast cancer cells following exogenous antigen loading or endogenous antigen processing. NT Not tested
| Number of effector cells tested per well | Frequencies of MP57-68-specific T cells as determined by IFN-γ secretion in ELISpot assaya | ||||
|---|---|---|---|---|---|
| Stimulib | |||||
| PBMC/p68 | PBMC/MP57-68 | KS24-mock/p68 | KS24/MP57-68 | KS24-M1 | |
| 10,000 | 0 | 101 | 0 | 76 | 27 |
| 3,000 | 0 | 31 | 0 | 26 | 11 |
| 1,000 | 0 | 12 | 0 | 12 | 5 |
| 300 | 1 | 5 | 0 | NT | 2 |
aT cells of a HLA-A*0201+ healthy donor were stimulated weekly using different stimulator cells. Frequencies of MP57-68-specific T cells were determined by IFN-γ ELISpot out of different MLTCs on day 14 using MP57-68-pulsed T2 cells as targets. T cells responding to the control peptide p68 are subtracted from T cells reactive to MP57-68
bT cells were stimulated respectively, with unpulsed and MP57-68-pulsed autologous PBMCs, unpulsed and MP57-68-pulsed IFN-γ–pretreated KS24 cells (mock transfectants), and IFN-γ–pretreated KS24 cells transfected with M1