Fig. 4.

Cytokine-conditioned medium from progressor HNSCC lines decreases CD80 costimulatory molecule expression. Regressor cell line B6C3-4scid was stimulated for 48 h with conditioned medium from regressor and progressor cell lines. Medium derived from the B7-clones, B7E3-4scid, and B7E11-4scid, which contained high levels of proinflammatory cytokines IL-1α, IL-6, and GM-CSF had a greater effect on reduction of CD80 expression (p=0.026 and p=0.019, respectively) as compared to medium from the regressor line B6D8-4scid (p=0.4624) that does not express proinflammatory cytokines. Result is representative of three independent experiments