Fig. 1.

ZNF692 was upregulated in osteosarcoma and associated with worse overall survival. (A) The expression profiles of ZNF692 in various tumors based on TIMER database. (B) The transcript level of ZNF692 in osteosarcoma tissues and adjacent normal tissues based on GSE126209 dataset. (C-E) ZNF692 expression was detected by western blot and qRT-PCR assay in osteosarcoma tissues and paired normal tissues. (F-G) ZNF692 protein level in osteosarcoma cell lines (HOS, MG63, U2OS, 143B, and Saos-2) and human osteoblast cells (FOB1.19) were detected by western blot assay, and quantitative analysis. (H) Univariate and multivariate Cox regression analyses were performed to evaluate the prognostic prediction ability of ZNF692 in osteosarcoma. (I) ROC curve analysis was performed to assess the accuracy of ZNF692 expression level, gender, and age in predicting patients’ prognosis in osteosarcoma. Student’s t-test and one-way ANOVA were performed to analyze differences between groups. All data are presented as means ± standard deviations (SD). *P < 0.05, **P < 0.01, ***P < 0.001