Fig. 2.

Silence of ZNF692 inhibits osteosarcoma cell proliferation, migration, and invasion. (A-C) Stably knocking down of ZNF692 was confirmed by qRT-PCR and western blot assays. (D-E) CCK-8 assay was performed to evaluate cell proliferation in cells stably knocking down of ZNF692 and control cells. (F-G) ZNF692 knock-down significantly impaired colony formation ability of osteosarcoma cells. (H) The proportion of Edu positive cell was decreased in osteosarcoma cells stably knocking down of ZNF692. Scale bar, 200 μm. (I-K) The migration ability of cells stably knocking down of ZNF692 and control cells was detected by wound healing assay (scale bar, 200 μm). (L-M) Representative images and quantitative analysis of cell invasion based on transwell assay. Student’s t-test and one-way ANOVA were performed to analyze differences between groups. All data are presented as means ± standard deviations (SD). *P < 0.05, **P < 0.01, ***P < 0.001