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. 2024 Apr 22;19:28. doi: 10.1186/s13062-024-00472-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Enforced expression of ZNF692 promotes cell proliferation, migration, and invasion. (A-C) Ectopic expression of ZNF692 was determined by qRT-PCR and western blot assays. (D-E) CCK-8 assay was performed to compare cell proliferation ability in cells stably overexpressing ZNF692 and control cells. (F-G) Representative images and quantitative analysis of colony formation in cells stably overexpressing ZNF692 and control cells. (H) The proportion of Edu positive cell was significantly increased in osteosarcoma cells stably overexpressing ZNF692. Scale bar, 200 μm. (I-K) Representative images and quantitative analysis of cell migration based on wound healing assay. Scale bar, 200 μm. (L-M) Enforced expression of ZNF692 increased cell invasion ability based on transwell assay. Student’s t-test and one-way ANOVA were performed to analyze differences between groups. All data are presented as means ± standard deviations (SD). *P < 0.05, **P < 0.01, ***P < 0.001