FIG. 7.
Replication of pBL19-BE ssDNA and dsDNA in the presence of wild-type AR1. Shown are Southern blots of DNA extracted from Xanthi protoplasts that were cotransfected with pBL19-2AE (wild type [WT]) and pBL19-BE or with pBL1-AR1fs8/Δ15-251 (ΔAR1) containing SqLCV-A with a frameshift mutation that results in a nonsense codon at residue 15 (31). DNA was digested with EcoRI. Blots were probed with an SqLCV-A- or SqLCV-B-specific probe, as indicated. −, not treated with mung bean nuclease (MN); +, treated with mung bean nuclease. No pBL19-BE ssDNA was replicated in the presence of the frameshift mutant pBL1-AR1fs8/Δ15-251 (data not shown).