Figure 8.

MgT-mediated protection against intestinal pathology and barrier dysfunction of APP/PS1 mice.

(A) Hematoxylin and eosin staining of representative colonic tissues from the three groups. Compared with the WT and MgT groups, the APP/PS1 group showed slight mucosal epithelial cell damage, hyperchromatic nuclear pyknosis, enhanced eosinophilia in the cytoplasm (black arrows), and slight inflammatory cell infiltration in the lamina propria (red arrows). (B) Western blotting and quantification of protein levels of ZO-1, occludin, and claudin-5 in the WT, APP/PS1, and MgT groups. Results presented as means ± SEM (n = 3), analyzed by one-way analysis of variance followed by Tukey’s honestly significant difference test; *P < 0.05, **P < 0.01, and ***P < 0.001 between the APP/PS1 and MgT groups. APP/PS1: Amyloid-β precursor protein and mutant human presenilin 1; MGT: magnesium-L-threonate; WT: wild-type; ZO-1: zona occludens 1.