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. 2024 Apr 22;20(4):e1011235. doi: 10.1371/journal.pgen.1011235

Fig 4. Intercellular communication between macrophages and epithelial cells via the SPP1 signaling pathway.

Fig 4

(A-B) Chord diagrams and bar graphs showing the interaction scores of various cell types in cancer predicted by the SPP1 signaling pathway. (C) Contribution of different receptor-ligand pairs of the SPP1 signaling pathway. (D) Expression of receptor-ligands in the SPP1 signaling pathway in different cell types. (E) Chordal graph showing interaction scores in different cell types via SPP1-CD44 receptor-ligand pairs. (F) Normalized enrichment score (NES) of different pathways in various cancers. (G) SPP1 levels in MDA-MB 231 (231)-induced TAM with SPP1 overexpression were detected by Western blot. (H) 231 cells were treated for 48 h with condition media from 231-induced TAM with SPP1 overexpression, or condition media (with SPP1 antibody or without) from 231-induced TAM. SOX2, CMYC, NANOG, OCT4 and PDL1 were detected by RT-qPCR. (I) 231 cells were treated for 48 h with condition media (with SPP1 antibody or without) from 231-induced TAM. The indicated proteins were detected by Western blot. Data were shown as mean ± SD and are representative of three independent experiments. P values were calculated using the 2-tailed 2-sample t test. *P < 0.05, **P < 0.01, ***P < 0.001.