Figure 1. TWEAK induces inflammatory activity in human keratinocytes relevant for AD.
(A-D) RNA-seq of human keratinocytes with triplicate cell cultures stimulated with human TWEAK, IL-13, or IL-22. (A-B) Venn diagram of upregulated transcripts that are AD specific (A) or common to both AD and psoriasis (B). (C-D) Heatmaps of a select subset of TWEAK upregulated transcripts that are also found upregulated in the skin lesions of AD patients categorized either as AD-specific transcripts (C) or commonly upregulated in AD and psoriasis (D). (E) Heatmaps showing the z-score transformed values of mean percentage of keratinocytes that were positive (i.e., have non-zero gene expression in single-cell data) for genes associated with TWEAK activity (left) and the average expression for each gene (right) from healthy controls, untreated AD patients, or dupilumab-treated AD patients. AD; Atopic dermatitis.
