Figure 2.

Quantification of the variability of passive membrane properties to evaluate the membrane-coated electrode performance under voltage-clamp mode of perforated patch clamp recordings. (A) Graphical diagrams of the recording configuration. The blue represents Escin, the perforating agent that creates chemical pores on the neuronal membrane underneath the electrode. (B) Comparison of the mean value of access resistance (Ra) during the membrane test with (+) or without (−) membrane coating, (C) Comparison of the standard deviation of Ra during the membrane test with (+) or without (−) membrane coating, (D) Comparison of the standard deviation of membrane capacitance (Cm) during the membrane test with (+) or without (−) membrane coating, (E) Comparison of the standard deviation of membrane resistance (Rm) during the membrane test with (+) or without (−) membrane coating, and (F) Comparison of the standard deviation of membrane time constant (Tau) during the membrane test with (+) or without (−) membrane coating. All data are obtained from the membrane test using the voltage-clamp mode of perforated patch clamp recordings from DN1p circadian clock neurons in Drosophila, by using standard patch-electrodes with (+) membrane-coated electrodes (N=12) and without (−) membrane coating (N=12). One DN1 neuron was recorded per fly. Single-electrode recordings were performed in random order between no coating and coating. Each inset (dashed line) shows a zoomed subplot. The statistics are Mann–Whitney U test with *p < 0.05, **p < 0.01, and ***p < 0.001.