Table 1.

Break and Reinitiation Points on NiV DI Genomes^a

DI Genome	Genome Breakpoint (nt)	Genome Reinitiation Point (nt)	Length (nt)	Overlap	DI Genome Classification	DI Genome Type	Virus Used to Generate DI Genome
DI-01	17170	17812	1512	0	Trailer copyback	Natural	rNiV-M C-
DI-03	16210	17953	2328	3	Trailer copyback	Natural	rNiV-M C-
DI-04	14776	16773	4944	1	Trailer copyback	Natural	rNiV-M C-
DI-05	16177	17827	2490	0	Trailer copyback	Natural	rNiV-M C-
DI-06	12657	17267	6570	0	Trailer copyback	Natural	rNiV-M
DI-07	17183	17840	1470	1	Trailer copyback	Natural	rNiV-M
DI-08	15715	15919	4860	0	Trailer copyback	Natural	rNiV-M
DI-09	16503	18046	1944	1	Trailer copyback	Natural	rNiV-M
DI-10	173	17932	486	2	Deletion	Natural	rNiV-M C-
DI-14	17428	18060	1014	0	Trailer copyback	Natural	rNiV-M C-
DI-15	17964	18152	378	0	Trailer copyback	Natural	rNiV-M D156
DI-16	17831	18112	546	5	Trailer copyback	Natural	rNiV-M D156
DI-18	16064	17716	2712	2	Trailer copyback	Natural	rNiV-M D156
DI-19	112	18143	216	0	Deletion	In silico	n/a
DI-20	250	17993	504	0	Deletion	In silico	n/a
DI-21	375	17872	750	0	Deletion	In silico	n/a
DI-22	499	17749	996	0	Deletion	In silico	n/a
DI-23	205	18147	306	0	Deletion	In silico	n/a
DI-24	98	17649	696	0	Deletion	In silico	n/a
DI-25	825	18040	1032	0	Deletion	In silico	n/a
DI-27	17289	17674	1530	1	Trailer copyback	Natural	rNiV-M D156
DI-28	16250	17050	3192	2	Trailer copyback	Natural	rNiV-M D156
DI-29	17633	17756	1104	1	Trailer copyback	Natural	rNiV-M D156
DI-30	14076	16554	5862	2	Trailer copyback	Natural	rNiV-M D156
DI-31	17511	17723	1260	0	Trailer copyback	Natural	rNiV-M D156
DI-32	16216	18123	2154	1	Trailer copyback	Natural	rNiV-M D156
DI-33	17706	17840	948	0	Trailer copyback	Natural	rNiV-M D156
DI-34	15218	15820	5454	2	Trailer copyback	Natural	rNiV-M/ZsG
DI-35	17797	18150	546	1	Trailer copyback	Natural	rNiV-M/ZsG
DI-36	15676	16971	3846	1	Trailer copyback	Natural	NiV-B
DI-38	807	18104	950	0	Deletion	Natural	rNiV-M D156
DI-dTom	112	18147	918	0	Minigenome	In silico	n/a
DI-BFP	112	18147	918	0	Minigenome	In silico	n/a
DI-gLuc	112	18147	918	0	Minigenome	In silico	n/a

Abbreviations: BFP, blue fluorescent protein; DI, defective interfering; dTom, dTomato; gLuc, Gaussia luciferase; n/a, not applicable; NiV, Nipah virus; nt, nucleotide.

^aNucleotide position based on antigenomic sense of NiV-M (GenBank accession no. AF212302). Overlap signifies number of nucleotides at junction that could come from either end of the genome. DI genome type: natural, found during passaging experiments; in silico, designed in silico.