Figure 9.

Validation of the gene expression with qPCR and proposed metabolic pathways in the liver regulated hepatic SHP deletion and overlaying IFNγ^−/−myeloid cells. A-B: Expression of selective transcripts indicated from bulk RNAseq analysis was validated with qPCR analysis (n = 6). Statistical analysis was performed with 2-way ANOVA with multiple comparisons. ∗, p < 0.05 for significant differences between Δhep_Con’ and fl/fl_Con’; #, p < 0.05 for significant differences between Δhep_Ifnγ^−/− and Δhep_Con’. The expression of genes in fl/fl_Con’ was used as the control. C. Summary for the pro-MASH pathways changed by hepatic SHP deletion and overlaying bone marrow-derived IFNγ^−/− myeloid cells in female and male mice. Pathways that were up- or down-regulated were denoted by the direction of the arrowheads. Pathways not found significantly to be changed in the current study were denoted by horizontal lines.