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. 2024 Apr 23;25:161. doi: 10.1186/s12859-024-05768-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Comparison of assignment percentages of real short E. coli reads from the three assemblies: INF32/16/A, INF13/18/A, and INF191/17/A by Mora, AugPatho2, and the initial aligner Pufferfish. The real abundance levels of the strains are represented by the red dots. The left subfigure shows the real abundances as red dots, which are at 2.5% 46.18%, and 51.20% respectively. This highlights that Mora has the smallest $l^{1}$ error. Mora assigns 3% of reads to INF32/16/A while AugPatho2 assigns 1% of reads to it. The right subfigure shows 10 of the other strains that had the highest assignments from Mora. AugPatho2 and Pufferfish assigns a lot of reads to a wrong strain